Data Sheet

Product Name: Normalized cDNA Library

Catalog No.: B1234035N                  LotNo.:B104114

Species:  Human

Tissue Type: Normal

Tissue Name: Brain tissue

Vector: BioExpress 1 (Not I—blunt, Eco RV)

Cells used for Electroporation: DH10B E. coli bacterial cells, T1 phage resistant

Diluent: 80% SOC:20%Glycerol

Titer: 5 x10⁹ cfu/ml

Average insert size: 1.8 kb

Percent recombinants: 96%

Actin Reduction: 130-fold reduction compared to the primary library

Storage conditions: -80º C

Primer(s) for sequencing:     M13FWD        GTA AAA CGA CGG CCA GT

M13REV        CAG GAA ACA GCT ATG AC

Donor Information:

Male: 23-27 year(s) old                                 Pool of 3 donor(s)

Female: _____________ year(s) old             Pool of _______ donor(s)

Pathological Diagnosis:

Components

1. 1 ml library

2. cDNA library user manual

3. Certificate of Analysis

4. Data Sheet

cDNA Library

Certificate of Analysis

Product Name: cDNA Library

Catalog Number: Bxxxxxxx

Shipping Condition: Dry Ice

Storage Condition: -70ºC

Shelf Life: Three year from the date of receipt under proper storage condition

Description

Our high quality cDNA libraries are constructed by using an oligo dT primer-adapter and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA from mRNA. After the second strand is synthesized, the double stranded cDNA is size fractionated, cloned directionally into our BioExpress vector and transformed into T1 phage resistant E. coli. Average insert size and insert size range are determined by restriction enzyme digestion of 24 clones picked at random from each library. The 4 kb BioExpress vector used for cloning is Puc based, confers ampicillin resistance and contains the CMV promoter for expression analysis. This vector also contains the SP6 and T7 RNA polymerase promoters flanking the MCS for RNA synthesis, the Amersham ET and M13 primer sites for sequencing and the F1 ori for single-stranded DNA production. By cloning the cDNA directionally into this vector the cDNA clones can be expressed, detected by antibody screening and the libraries can be used to produce normalized or subtracted libraries.

Quality Control

1. Minimum number of primary clones: 10⁶

2. Minimum percentage of recombinant clones: 87%

3. Minimum insert size: 1 kb

4. Average number of primary clones: 3x10⁶

5. Average percentage of recombinant clones: 95%

6. Average insert size: 1.5 kb