| Regular TAQ, with a two-dye reaction buffer
Regular TAQ, with a two-dye reaction buffer
Features
• Excellent price/performance
• Easy visual recognition
• Direct loading onto agarose gels
• Robust performance
• Available as a ready-to-use 2x reaction mix (MangoMix)
Applications
• High throughput applications
• Suited to a wide range of PCR assays
• Products suitable for TA cloning
Description
MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase which offers consistent results across a wide range of DNA templates. MangoTaq DNA Polymerase possesses 5’-3’ exonuclease activity and leaves an ‘A’ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. The polymerase is supplied with two different reaction buffers for greater user flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.
The two reaction buffers supplied are: 5x Colored reaction buffer and 5x Colorless reaction buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis, without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist.
Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection.
% Agarose Gel Red Dye Orange Dye
0.7 1.5kb 100bp
1.0 750bp 25bp
1.5 500bp 10bp
2.0 300bp <10bp
3.5 75bp <10bp
Reaction Conditions (for a 50µl volume)
5x MangoTaq Reaction buffer
(Colored or colorless) 10µl
50mM MgCl2 Solution 1.5-4.0µl
100mM dNTP Mix (see below) 0.5-1.0µl
Template and Primers as required
Enzyme 0.5-1.0µl
Water (ddH2O) up to 50µl
Denature: 94-96°C
Elongate: 70-72°C (allowing 15-30 seconds/ Kb)
The specificity and performance of MangoTaq DNA Polymerase can be further improved with the use of 2x PolyMate Additive (Cat No. BIO-37041), which is designed for GC or AT-rich DNA, “dirty” templates or sequences with a high level of secondary structure.
This data is intended for use as a guide only; conditions will vary from
reaction to reaction and may need optimization.
Concentration: 5u/µl
Components 1000 Units 2000 Units 5000 Units 20000 Units
MangoTaq DNA Pol. 200µl 2 x 200µl 5 x 200µl 20 x 200µl
5x MangoTaq Colored
Reaction Buffer 4 x 1.5ml 8 x 1.5ml 20 x 1.5ml 80 x 1.5ml
5x MangoTaq Colorless
Reaction Buffer 4 x 1.5ml 8 x 1.5ml 20 x 1.5ml 80 x 1.5ml
50mM MgCl2 Solution 2 x 1.2ml 4 x 1.2ml 10 x 1.2ml 40 x 1.2ml
Reagent Specifications:
MgCl 2 Stock Solution: 50mM MgCl2 (suggested final concentration 1.5mM - 4mM).
Storage Buffer:
20mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% Glycerol, and stabilizers.
Storage Conditions:
MangoTaq can be stored for 12 months at -20°C.
Shipping Conditions:
On Dry Ice or Blue Ice
Unit Definition:
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
Associated Activities:
Endonuclease and exonuclease activities were not detectable after 2 and 1 hour incubations, respectively, of 1µg lambda DNA and 0.22 µg of EcoR I-digested lambda DNA at 72°C in the presence of 15-20 units of MangoTaq DNA polymerase.
Associated Products:
Product Name Pack Size Cat No
dNTP Set 4 x 25µmol BIO-39025
dNTP Mix 100mM total 1 x 500µl BIO-39028
MangoMix 250 Reactions BIO-25033
SureClean Plus 1 x 5ml BIO-37047 |