Indo-1,AMM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127 (See 59000 and refs. Therein), a mild detergent, is often used as a dispersing agent to facilitate the loading.Indo-1/AM itself does not bind Ca²⁺, but it is readily hydrolyzed to indo-1 by endogenous esterases once the dye is inside the cells.

• Light yellow solid soluble in DMSO.
• Keep frozen at -20?C.Protect from light, especially when in solution.
• C₄₇H₅₁N₃O₂₂
• Mwt: 1010.
• [112926-02-0].