Catalogue Numbers:
ImmoMix™ 100 Reactions BIO-25019
ImmoMix™ 500 Reactions BIO-25020
ImmoMix™ Red 100 Reactions BIO-25021
ImmoMix™ Red 500 Reactions BIO-25022
Features:
• Same high-specificity and performance as IMMOLASETM DNA Polymerase
• Enhanced specificity and reduced background
• Convenient pre-mixed, pre-optimized 2x solutions
• Reduced risk of contamination
• Dramatically decreases the time required for reaction set-up
•
Reproducible results
Applications:
• Highly suited to real-time assays
• Ultra-high specificity for multiplex reactions
•
TA cloning
Description:
ImmoMix is a complete ready-to-use heat-activated 2x Reaction-Mix, which simply requires the consumer to add only water, template and primers, and then pre-heat to 95°C for 7 minutes to successfully carry out PCR assays. The 7-minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, by ensuring the enzyme is inactive at initial low temperatures.
ImmoMix Red combines all of the features and advantages of ImmoMix, and contains an additional inert red dye. This non-toxic, non-hazardous red dye allows users to load samples directly onto a gel, without the need to add loading buffer since the mix is of sufficiently high density to sink to the bottom of the gel. Adequate mixing is also ensured when reactions are set up.
ImmoMix and ImmoMix Red are based on our IMMOLASE™ DNA polymerase, which leaves an ‘A’ overhang, and have been optimised for a wide variety of templates. An additional 50mM of MgCl2 solution is included should any fine adjustments be required.
ImmoMix and ImmoMix Red dramatically reduce the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to pipetting errors.
Product Citations:
1. Fakhari, F.D.,
et al. The Journal of Clinical Investigation 116(3), 735-742 (2006).
2. Hugo, E.R
et al. Endocrinology 147(1), 306-313A (2006).
3. Dobolyi, A., et al. Comperative Neurobiology, 498(3), 375-389 (2006).
Notes:
1. IMMOLASE is a Trademark of Bioline.
2. Purchase of this product does not convey a licence to perform any patented process.
3. This product insert is a declaration of analysis at the time of manufacture.
4. Research Use Only.
Product Specifications:
Batch details:
Batch No: See vial
Storage Conditions:
ImmoMix and ImmoMix Red can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freez /thaw cycles should be avoided.
Shipping Conditions:
On Dry-Ice or Blue-Ice
Composition of 2x ImmoMix:
IMMOLASE™ DNA Polymerase
32mM (NH4)2SO4
134mM Tris-HCl (pH 8.3 at 25°C)
0.02% Tween 20
2mM dNTPs
Stabilizer
3mM MgCl2
Additional reagents supplied:
MgCl 2 Stock Solution: 50mM MgCl2
Extended Stability: A sample of ImmoMix and ImmoMix Red were stored at +4°C over a five-week period and tested daily. No detectable loss of activity was evidenced. However, in view of the possibility of microbial contamination, please adhere to the recommended storage conditions.
|
Associated Products:
Product Name |
Pack Size |
Cat No |
|
dNTP Set |
4 x 25µmol |
BIO-39025 |
|
dNTP Mix 100mM total |
1 x 500µl |
BIO-39028 |
|
40mM total |
1 x 500µl |
BIO-39043 |
|
ImmoMix 100 |
Reactions
500 Reactions |
BIO-25019
BIO-25020 |
|
HyperLadder I |
200 Lanes |
BIO-33025 |
|
|
500 Lanes |
BIO-33026 |
|
Agarose |
500g |
BIO-41025 |
ImmoMix and ImmoMix Red Protocol
The Bioline ImmoMix and ImmoMix Red preparations are designed with ease-of-use in mind. Each reaction requires 25µl of 2x ImmoMix (supplied) in addition to Primers and Template, and 18.2 mO water for a final reaction-volume of 50µl.
|
Reaction Conditions (For a 50µl reaction)
ImmoMix / ImmoMix Red |
25µl |
|
Template and Primers |
as required |
|
Water (ddH2O) |
up to 50µ |