CSI 007-01 can be used for detection of collagens by ELISA. CSI 007-01 has been used successfully for

immunohistology on frozen unfixed sections of human (3) and kangaroo (4) skin, and of new dog tissue

associated with biomaterial implants (2,5). If fixation of tissue is required, acetone or ethanol is

recommended.

REACTIVITY

IMMUNIZATION Female SJL/J mice immunized i.p.

Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the

connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk

of rupture of major arteries or the intestine.

ANTIGEN

CULTURE MEDIUM RPMI 1640 with 10% fetal calf serum

PRESENTATION

IMMUNOGEN Acid-digested pepsin soluble human type III collagen

Content: Available in 200 µL and 1 mL, 1 mg/mL

Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Storage: In the dark at 4-8ºC

Preparation: Protein-A/G purified

FUSION PARTNER NS1/1.Ag4.1.

EPITOPE SPECIFICITY

APPLICATION

1. Werkmeister JA, Ramshaw JAM (1991) Multiple antigenic determinants on type III collagen. Biochem J

274:895-898.

2. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for

assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

3. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of

human skin. Acta Derm Venereol 69:399-402.

4. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo

skin. Das Leder 42:41-44.

5. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of

long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119.