Apolipoprotein B-100 0.1 mg Apolipoprotein B-100 0.05 mg

Source: Chicken

 Purity: Immunoaffinity Purified

 Clonality: Polyclonal

 Crossreactivity: Human

 Format: Phosphate-Buffered Saline. No preservatives added.

 Storage: 4 degree C for short term (weeks) and -20 degree C for long term. Avoid frequent freeze and thaw.

 Stability: 6-12 months at -20 degree C.

 Shipping: Products may be shipped on ice pack or dry ice. 
 
 APOB ANTIBODY TARGET DESCRIPTION:
Synonym Names for APOB antibody: APOB; FLDB; Apo B-100; Apolipoprotein B

Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Subcellular Location: Secreted protein.

Ptm: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.

Rna Editing: Modified_positions=2180; Note=The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.

Disease: Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB-100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100].

Disease: Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors.

Disease: Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Similarity: Contains 1 vitellogenin domain.

Summary: Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of mature apoB-100. Apolipoprotein B-48, a shortened form of apoB-100 lacking the LDL-receptor region, is a product generated when a stop codon (UAA) at residue 2180 is created by RNA editing.

Apolipoprotein B-100 reacts with human.

OMIM: 107730; gene+phenotype. [NCBI / EBI]
144010; phenotype. [NCBI / EBI]

Pathways:
Reactome Event:Lipid metabolism 73923

Products similar to APOB antibody:
 IgG
    Apo B [1609] antibody, IgG
    APOBEC3G (apolipoprotein B mRNA editing enzyme. catalytic polypeptide-like 3G), IgG
    DNA dC->dU-editing enzyme APOBEC-3G, IgG
    Apo B [1606] antibody, IgG
    Apo B antibody, IgG
 IgY
    Apolipoprotein B-100 (Biotin Conjugated), IgY
 
 
SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more. 
 
 APPLICATIONS for APOB ANTIBODY:
 ELISA, WB: Tested
 ICC, IHC: Not Tested
 
 
 TESTING: (secondary reagents and protocols )
 ELISA: Serum protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY-HRP as 2nd antibody. Fixed amount of Antibody (1ug/ml) and serial dilutions of antigen. ( anti-APOB )