Features:
• Convenient pack containing 4 separate 100mM solutions
• >99% pure by HPLC
• Extended shelf-life of 24 months at -20°C
• Free from PCR inhibitors
• DNase, RNase and Nickase free
• Manufactured by Bioline in a purpose-built laboratory
• Custom, Bulk and OEM Nucleotides service
Applications:
Suitable for a wide variety of applications such as:
• Standard PCR assays
• Long range PCR assays
• cDNA synthesis
• qPCR
• Microarrays
• DNA sequencing
• Mutagenesis
• Genotyping
• DHPLC
• Labeling
Description:
Ultra-Pure dNTPs Bioline Ultra-pure dNTPs are enzymatically synthesized from premium quality raw materials, using highly specific production systems in our purpose built facilities. The manufacturing process eliminates impurities and PCR-specific inhibitors such as modified nucleotides, tetraphosphates and pyrophosphates commonly observed in other commercially available dNTP products. Bioline dNTPs are purified with quantitative HPLC and possess at least 99% purity. Improved Stability and Extended Shelf-Life All Bioline dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelf-life due to the bacteriostatic activity of Lithium towards various microorganisms.
Characteristics:
dATP dCTP
Product dATP Lithium 100mM Solution dCTP Lithium 100mM Solution
Nomenclature 2'-deoxyadenosine-5'-triphosphate 2'-deoxycytidine-5'-triphosphate
Formula C10H12Li4N5O12P3 C9H12Li4N3O13P3
Molecular Weight 514.9g/mol 490.9g/mol
?max pH 7.0 259nm 272nm
e at ?max 15.4 E x mmol-1 x cm-1 9.1 E x mmol-1 x cm-1
A250/A260 0.78 ± 0.03 0.82 ± 0.03
A280/A260 0.15 ± 0.02 0.98 ± 0.03
Concentration 100mM ± 2% 100mM ± 2%
Appearance Clear Colourless Solution Clear Colourless Solution
pH of Solution 7.5 7.5
dNTP (HPLC Area) = 99% = 99%
dNDP (HPLC Area) < 1% < 1%
DNases, RNases, Nicking Activity --------- Negative----------------------
dGTP dTTP Product dGTP Lithium 100mM Solution dTTP Lithium 100mM Solution
Nomenclature 2'-deoxyguanosine-5'-triphosphate 2'-deoxythymidine-5'-triphosphate
Formula C10H12Li4N5O13P3 C10H13Li4N2O14P3
Molecular Weight 530.9g/mol 505.9g/mol
?max pH 7.0 252nm 267nm
e at ?max 13.7 E x mmol-1 x cm-1 9.6 E x mmol-1 x cm-1
A250/A260 1.16 ± 0.05 0.65 ± 0.03
A280/A260 0.66 ± 0.03 0.73 ± 0.02
Concentration 100mM ± 2% 100mM ± 2%
Appearance Clear Colourless Solution Clear Colourless Solution
pH of Solution 7.5 7.5
dNTP (HPLC Area) = 99% = 99%
dNDP (HPLC Area) < 1% < 1%
DNases, RNases, Nicking Activity------Negative-----------------------
Product Insert
100mM dNTP Set
Product Specifications:
A set of 4 separate 100mM lithium salt solutions (dATP, dGTP, dCTP, and dTTP, (pH 7.5). Each solution contains 25µmol (250µl) of the corresponding dNTP.
Batch details:
Concentration: 100mM each
Presentation: 250µl per tube
Batch No: See vial
Storage and Stability:
Bioline dNTPs can be stored at -20°C or -70°C, in a constant-temperature freezer for 24 months. Avoid multiple freezing/thawing. Bioline dNTPs will remain stable if stored as specified. For long-term usage, aliquoting is recommended.
Shipping Conditions:
On Dry-Ice or Blue-Ice
Characteristics: See table bellow for characteristics of each dNTP.
dNTP Master Mix Preparation
Prepare a Master Mix for DNA synthesis as follows: Mix equal volumes of all separate dNTP solutions in a new micro-centrifuge tube. The final solution has a concentration of 25mM of each dNTP, which corresponds to a 100x working concentration.
Product Citations:
1. Tabone, T., et al. Nucleic Acids Research, 34(6), e45, 2006.
2. Dean, Y.D., et al. Biol. Chem. 275(44), 34382-34392, 2000.
3. Lloyd, R.E., et al. Genetics, 172, 2515-2527, 2006. |