Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM.^1-4 Renilla luciferase, encoded by the luc gene, has been widely used as a repoter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer(BRET)⁵ to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.^6-9

• Yellow solid soluble in MeOH or EtOH
• Store at -20?C and protect from light
• C₂₆H₂₁N₃O₃
• Mwt: 423
• [55779-48-1]