Cultrex® Basement Membrane Extract PathClear®
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Basement Membrane Extract can be used for promotion and maintenance of a differentiated phenotype in a variety of cell cultures including primary epithelial cells, endothelial cells, and smooth muscle cells. It has been employed in angiogenesis assays, tumor cell invasion assays, and as a vehicle to augment the tumorigenicity of injected tumor cells in nude mice.
The PathClear® designation means that in addition to standard sterility, endotoxin and MAP testing, the BME is tested by PCR and is clear of 31 pathogens and viruses, including LDEV. Each lot is rigorously qualified in biological performance assays. PathClear® BME is available in normal and reduced growth factor form, with and without phenol red, and is ideal for in vivo murine research work and other work requiring BME free from viruses, bacteria and mycoplasma.
This product is made and marketed under patent license from the United States Public Health Service. Ref. U.S. Patent 4,829,000 issued May 9, 1989 and U.S. Patent 5,158,874 issued October 27,1992, all
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| Applications: |
The many applications for Cultrex Basement Membrane Extract, require different thicknesses and concentrations. Examples include use of diluted extract at <5 mg/ml for support of primary cell propagation; higher concentration >5 mg/ml for cell propagation and differentiation studies. Basement Membrane Extract is ideal for Tube Assay, Ring Assay, cell invasion studies, in vivo and in vitro angiogenesis assays, tumorgenicity and 3D Culture Assays. |
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| Source: |
Murine Engelbreth-Holm-Swarm (EHS) tumor |
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| Storage: |
Store at -20°C or colder in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
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| Related Articles: |
Fridman, R., Kibbey, M.C., Royce, L.S., Zain, M., Sweeny, T.M., Jicha, D.L., Yannelli, J.R., Martin, G.R., Kleinman, H.K. 1991. Enhanced Tumor Growth of Both Primary and Established Human and Murine Tumor Cells in Athymic Mice afer Coinjection with Matrigel. Journal of the National Cancer Institute. 83:769-774. | |