Regular TAQ, Red

Features
• Easy visual recognition
• Direct loading onto agarose gels
• Same high performance as BIOTAQ™ DNA Polymerase
• Leaves ‘A’ overhang
• Available as a ready-to-use 2x reaction mix (BioMix™ Red)

Applications
• Routine PCR assays
• Products suitable for TA cloning
• High throughput applications

Description
BIOTAQ™ Red DNA Polymerase is a formulation of our regular BIOTAQ DNA Polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing. When the reaction is complete, a sample of the reaction mix can be loaded directly onto the agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis.
The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5 Units per 50µl reaction.
The specificity and performance of BIOTAQ Red can be further improved with the use of 2x PolyMate Additive (Cat No. BIO-37041), which is designed for GC or AT-rich DNA, “dirty” templates or sequences with a high level of secondary structure.
BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.

Reaction Conditions (for a 50µl volume)
10x NH4 Buffer                           5µl
50mM MgCl2 Solution                1.5 - 4.0µl
100mM dNTP Mix (see below)   0.5 - 1.0µl
Template and Primers                  as required
BIOTAQ Red                             1.0 - 2.5µl
Water (ddH2O)                          up to 50µl

Concentration: 1u/µl

Components:
BIOTAQ Red DNA Polymerase  100 Units  500 Units  2500 Units
BIOTAQ Red DNA Polymerase  100µl         500µl        5 x 500µl
10x NH4 Reaction Buffer             1.2ml       2 x 1.2ml    10 x 1.2ml
50mM MgCl2 Solution                 1.2ml          1.2ml       5 x 1.2ml

Reagent Specifications:
10x NH 4 Reaction Buffer: 160mM (NH4)2SO4, 670mM Tris-HCl (pH 8.8 at 25°C), 0.1% stabilizer
MgCl 2 Stock Solution: 50mM MgCl2 (suggested final concentration 1.5mM – 4mM).

Storage Buffer:
20mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% Glycerol, stabilizers and inert dye.

Storage Conditions:
BIOTAQ Red can be stored for 12 months at -20°C.

Shipping Conditions:
On Dry Ice or Blue Ice

Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.

Associated Activities:
Endonuclease and exonuclease activities were not detectable after 2 and 1hour incubations, respectively, of 1µg lambda DNA and 0.22 µg of EcoR I-digested lambda DNA at 72°C in the presence of 15-20 units of BIOTAQ™ Red DNA polymerase.

Associated Products:
Product Name              Pack Size     Cat No   

dNTP Mix                     500µl       BIO-39028
2x PolyMate Additive   2 x 1.2ml   BIO-37041
Hyper Ladder I            200 lanes   BIO-33025
Agarose                         100g       BIO-41026