Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3??end terminal labeling.¹ The terminal deoxynucleotidyl transferase (TDT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.^3,4 The number '16' is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP (#40029 and #40029-1) and biotin-20-dUTP (#40030 and #40030-1). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, the longer the linker, the better biotin can interact with avidin or streptavidin.

• Colorless solution
• Store at -20?C and protect from light
• C₃₂H₄₈N₇O₁₈P₃Sli₄
• MWt: 971.5