M14328

DESCRIPTION
Small interfering RNA (siRNA) is a short double strand RNA, which hybridizes to the target RNA and directs target degradation by enzymatic cleavage. Vicgene provides siRNA products targeting hundreds of different genes. To quantitatively analyze gene silence efficiency,we also provides gene expression quantitative kits to the corresponding siRNA products.
In the assay, FAM labeled probes hybridize to the amplicon between the forward and reverse primers and are cleaved by Tag DNA polymerase during the extension step.
Cleavage of the probes separate the FAM dye from the quencher generating a fluorescent signal proportional to the number of amplicons produced.


PRODUCTS
Gene Expression Quantitative Kits are TagMan based assays. Each kit is sufficient for 250 reactions
at a 20µL reaction volume.

STORAGE
Store at -20° C; stable for six months from the date of shipment.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

PROTOCOL
1. PCR Reaction
Master Mix    10 ul
Assay Mix       1 ul
cDNA             9 ul
Total             20 ul

2. Thermal Cycle Condations
Initial Setup     95°C      10 min
Each of 40 Cycles
Denature          95°C      15 sec
Anneal/Extend  60°C      1 min