ANEPPS dyes are fast responding membrane potential dyes.¹ Biotium offers both Di-4-ANEPPS and Di-8-ANEPPS. Di-4-ANEPPShas the problem of being internalized in cells over long-term, so it is mainly used for short-term experiments. However, because Di-8-ANEPPS is more lipophilic, the dye is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS.^2,3

• l_ex\l_em(MeOH) = 498 nm / 713 nm.
• Orange red solid soluble in DMF, ethanol or DMSO.
• Store at 4?C and Protect from light, especially when in solution.
• C₃₆H₅₂N₂O₃S.
• Mwt:593