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Anti-Human PPM1D/WIP1 Monoclonal
Antibody Cat# 2380-MC-100
Anti-Human PPM1D/WIP1 Monoclonal Antibody (Clone WC10) is a serine/threonine
phosphatase that is a transcriptional target of p53 and is implicated in cell cycle control,
spermatogenesis, and lymphoid cell function. PPM1D/WIP1 is known to directly inhibit
phosphorylation of the p38 mitogen-activated (MAP) kinase protein. PPM1D/WIP1 mRNA is
rapidly induced in response to UV damage and the protein localizes to the nucleus.
PPM1D/WIP1 null mice have increased susceptibility to pathogens and reduced male fertility
and longevity.
Immunogen: Synthetic peptide corresponding to amino acids 40 to 59 of the human
PPM1D/WIP1 protein sequence.
Specificity: Anti-PPM1D/WIP1 is specific for human PPM1D/WIP1. Under certain
conditions, a ~50 kDa band is also detected in human samples. Not recommended for
detection of mouse PPM1D/WIP1.
Preparation: This antibody is provided as purified immunoglobulin from mouse ascites in
1X PBS without preservative.
Application: Western blotting (Figure 1), immunoprecipitation, and immunochistochemistry.
For both Western blotting and immunoprecipitation a 1 µg/ml dilution is recommended.
For immunochistochemistry the recommended concentration is 3 µg/ml. Empirical testing
will be required for optimal results.
Storage: Store at -4°C.
Reference:
1. Choi, J., B. Nannenga, O.N. Demidov, D.V. Bulavin, A. Coney, C. Brayton, Y. Zhang, I.N. Mbawuike, A. Bradley, E. Appella, and
L.A. Donehower. 2002. Mice deficient for the wild-type p53-induced phosphatase gene (Wip1) exhibit defects in reproductive
organs, immune function, and cell cycle control. Mol. Cell. Biol. 22:1094-1105.
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