HT PAR Sandwich ELISA measures net PAR levels in extracts from peripheral blood mononuclear cells that are regulated by endogenous PARP and PARG activities. Cellular PAR and PAR proteins are captured by a PAR Monoclonal antibody bound to the wells of a 96 well plate. Incubation with a polyclonal probe PAR antibody, followed by goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlate with the amount of cellular PAR. This assay is ideal for quantification of PAR in peripheral blood mononuclear cells and tissue culture cells, for monitoring the efficacy of PARP inhibitors on cellular PAR formation, and for verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/ anticancer drug combination therapy.

The assay is Chemiluminescent and measures sensitivity down to 10 pg/ml of PAR.