Ready-to-go pre-mix

Datasheets

BioMix™

Features

·         Convenient pre-mixed, pre-optimized 2x solutions

·         Reduced risk of contamination

·         Dramatically decreases the time required for reaction set-up

·         Reproducible results

Applications

·         Routine PCR applications

·         Products suitable for TA cloning

·         High throughput

Description

BioMix™ is a complete ready-to-use 2x reaction mix containing an ultrastable Taq DNA polymerase. Developed to perform PCR assays of many common genomic and cDNA templates, the user has simply to add water, template and primers.

BioMix dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to errors.

BioMix has been optimized for a wide variety of templates, however an additional 50mM of MgCl₂ solution is included should any fine adjustments be required.

BioMix Protocol

Reaction Conditions (For a 50µl reaction)

Denature: 94-96°C

Elongate: 70-72°C (allowing 15-30 seconds/kb)

For optimal resolution of PCR products, we recommend the use of Tris-Acetate EDTA (TAE) buffer for gel preparation and electrophoresis.

An additional tube of 50mM MgCl2 is provided should any fine adjustments be necessary. The table below shows the volume of MgCl2 that must be added to a 50µl final reaction volume to achieve the desired final concentration.

This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.

Product Specifications

Batch details:

Batch No: See vial

Cat No: See vial

Components

Reagent Specifications:

MgCl2 Stock Solution: 50mM MgCl2

Storage Conditions:

BioMix and BioMix Red can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.

Shipping Conditions:

On Dry Ice or Blue Ice

Associated Products:

Concentration

2x

Product Citations

1. Hancock, M.K., et al. J. Biol. Chem 277(49), 47205-47212 (2002).

2. Prado, M., et al. Electrophoresis 26(15), 2918-2926 (2005).

3. Reddy, S.T., et al. J. Biol. Chem 279(37), 38658-38667 (2004)