5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other biomolecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. See Figure 7.4 for spectra.
- lex\lem = 495/519 nm.
- Yellow solid soluble in DMSO or DMF.
- Store at -20?C and protect from light.
- C25H15NO9.
- Mwt: 473.4.
- [117548-22-8]