DAPI is a popular blue fluorescent DNA probe. The dye binds to the minor groove of dsDNA with a ~20-fold fluorescence enhancement. See Figure 9.3 for spectra.
- lex\lem = 358/461 nm (DNA).
- Off-white solid soluble in H2O.
- Store at 4?C and protect from light, especially in solution.
- C16H17Cl2N5.
- Mwt. = 350.25.
- [28718-90-3]