Description
The FavorPrep™ Plasmid Extraction Midi and Maxi Kit provides pre-packed an ion exchange resin column for high-purity plasmid DNA maxiperp from 50 - 300 ml bacterial cultures. Depending on the copy-number of plasmids, either a 50-150ml (low-copy plasmid) or a 150 - 500 ml (high-copy number) bacterial suspension could be used in the process. When using the standard protocol, the entire midi prep process can be completed in 120 minutes or less and up to 500 µg of plasmid can be expected. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications.

Features
•  Purity: equity to that obtained by 2x CsCl-gradient centrifugation .
•  Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.
•  Time saving: Complete the process in less than 120 minutes.

Applications
The purified plasmid DNA can be immediately used in any downstream molecular biology application.
•  Transfecttion
•  Microinjection
•  Sequencing
•  PCR
•  Restriction enzyme digestion

Operation Time
Less than 120 minutes

Operation Format
Gravity-Flow

Agarose Gel Analysis Of Plasmids Purified

Agarose gel analysis of various plasmid DNA purified with Plasmid DNA Midi / Maxi Kit
C: crude lysate
F: flow-ghrough
W: wash
E: elute
M: 1Kb DNA Ladder

Plasmid pBluescript (high-copy) and pBR322 (low-copy) are purified with the Plasmid DNA Midi / Maxi Kit. DNA in crude lysate from alkaline lysis and fractions from each gravity-flow step was collected by isopropanol precipitation and load to each lane.

Applications
•  Transfecttion
•  Microinjection
•  Restriction enzyme digestion
•  Sequencing
•  PCR

Procedure


In the process, the modified alkaline lysis method and RNase treatment are used to get cleared cell lysate plasmid DNA in crude lysate has been bound to the column, the contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120minutes without ultracentrifuges and HPLC or other toxic reagents.

Storage Conditions
FavorPrep™ Plasmid DNA Extraction Kit can be stored at room temperature (15-25 ? ). After adding RNase A, PDE Buffer1, should be stored at 4 ? and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ? ).