All reagents for polymerase reactions

Features
           • Ideal for setting up new procedures
           • Designed for easy optimizations of PCR applications
           • Contains ultra-pure dNTPs manufactured by Bioline
           • Supplied with 2x PolyMate Additive for difficult or “dirty” templates

Applications
           • Routine PCR applications
           • Products suitable for TA cloning

Description
The BIOTAQ™ PCR Kit contains all the necessary components to perform PCR assays on a wide range of DNA templates. In addition to dNTPs, the PCR Kit is based on our widely used BIOTAQ DNA Polymerase, which achieves dependable results.
BIOTAQ DNA Polymerase is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5’-3’ exonuclease activity and leaves an ‘A’ overhang such that the primer extension product is suitable for effective integration into TA cloning vectors.

Kit Components
BIOTAQ DNA Polymerase                        Size
BIOTAQ™ DNA polymerase @ 5u/µl     500 Units
10x NH4 Buffer                                        2 x 1.2ml
50mM MgCl2 Solution                                 1.2ml
10mM dNTP Mix                                           1ml
2x PolyMate Additive                                   1.2ml

Reagent Specifications:
10x NH 4 Reaction Buffer: 160mM (NH4)2SO4, 670mM Tris-HCl (pH 8.8 at 25°C), 0.1% stabilizer
MgCl 2 Stock Solution: 50mM MgCl2 (suggested final concentration 1.5mM – 4mM).
10mM dNTP Mix: Ultra-pure dNTPs are manufactured by Bioline and consist of a mix of dATP, dGTP, dCTP, and dTTP as Lithium salts (pH 7.5).
2x PolyMate Additive: Provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT-rich DNA, repetitive sequences or sequences with a high level of secondary structure. PolyMate acts as a melting agent which enables the DNA polymerase and oligonucleotides to provide greater access to the template DNA.

Product Insert
BIOTAQ™ PCR Kit

Storage Buffer:
20mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% Glycerol, and stabilizers.

Storage Conditions:
BIOTAQ PCR Kit can be stored for 12 months at -20°C.

Shipping Conditions:
On Dry Ice or Blue Ice.

Associated Activities:
Endonuclease and exonuclease activities were not detectable after 2 and 1-hour incubation, respectively, of 1µg lambda DNA and 0.22 µg of EcoR I digested lambda DNA, respectively at 72°C in the presence of 15-20 units of BIOTAQ™ DNA polymerase.

Unit definition:
One unit is defined as the amount that incorporates 10nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.

Associated Products:
Product Name         PackSize         Cat No
Hyper Ladder I     200 Lanes      BIO-33025
HyperLadder IV   200 Lanes       BIO-33029
Agarose                    100g          BIO-41026

Product Citations:
1. Melo, M., & Hansson, B. Molecular Ecology Notes 6(4), 1266 (2006).
2. Robba, L., et al. American Journal of Botany 93,1101-1108 (2006).
3. Rens, W., et al. Nature Protocols 1, 783-790 (2006).