Description705-FLP plasmid is designed for use in FLP-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at 30°C. The plasmids will not propagate and will get lost when incubated at 37°C.

The expression of the FLP-recombinase is driven by the thermosensitive promoter cI578 (?PR promoter). Therefore, the expression of Cre is repressed at 30°C and induced between 37-42°C. The plasmid carries a chloramphenicol resistance.

Note: The sequence of 705-FLP was compiled from information found in sequence databases, published literature, and other sources, together with partial sequences obtained by Gene Bridges. This vector has not been completely sequenced. The digestion patterns for BamHI, EcoRI, HindIII, PstI, XbaI and XhoI are indicated.

Contents705-FLP:

expression plasmid for FLP recombinase (0.2 ug/ul, 20 ul)
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