TAQ subsititute, economical

TAQ subsititute, economical

Features
      • Fast amplification (up to 6Kb per minute)
      • Amplifies fragments up to 8Kb
      • Active at a wide range of Mg concentrations

Applications
       • Taq substitute for routine applications
       • Products generated are suitable for TA cloning

Description
HybriPol™ is a novel thermostable DNA Polymerase, which has been synthesized through the recombinant fusion of two polymerase domains. HybriPol is a Taq substitute, and is highly suited to routine PCR applications.
HybriPol possesses 5’-3’ polymerase activity and can be substituted in protocols that currently use Taq polymerase, without the need to modify the protocol. As compared with Taq polymerase, Hybripol exhibits a DNA elongation rate, which is 1.5 times higher than Taq and an affinity for long templates (>2500 bp) at least 3 times higher than Taq, resulting in robust yield within a short reaction time. Hybripol can synthesize DNA fragments up to 8Kb (Lambda DNA as a template) with the same fidelity as Taq polymerase. HybriPol leaves an A’ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
Reaction Conditions (for a 50µl volume)
10x Reaction Buffer                            5µl
50mM MgCl2 Solution                1.5 – 4.0µl
100mM dNTP Mix (see below)   0.5 - 1.0µl
Template and Primers                  as required
Enzyme                                       0.5 – 1.0µl
Water (ddH2O)                            up to 50µl

Denature: 94-96°C
Elongate: 70-72°C (allowing 10-30 seconds/Kb)
This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.
Specificity and performance of HybriPol can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC- or AT-rich DNA, “dirty” templates or sequences with a high level of secondary structure.

Concentration: 5u/µl

Components
HybriPol DNA Polymerase   1000 Units         2500 Units
HybriPol DNA Polymerase      200µl              5 x 100µl
10x Reaction Buffer              4 x 1.2ml            10 x 1.2ml
50mM MgCl2 Solution            1.2ml                 5 x 1.2ml

Storage Conditions:
HybriPol can be stored for 12 months at -20°C.

Shipping Conditions:
On Dry ice or Blue ice.

Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.

Associated Activities:
Endonuclease and exonuclease activities were not detectable after 2 and 1 hour incubations, respectively, of 1µg lambda DNA and 0.22µg of EcoR I-digested lambda DNA at 72°C in the presence of 15-20 units of HybriPol™ DNA polymerase.

Associated Products:
Product Name            Pack Size     Cat No
dNTP Mix                    500µl      BIO-39028
2x PolyMate Additive  2 x 1.2ml  BIO-37041
HyperLadder I           100 Lanes  BIO-33053
Agarose                        100g       BIO-41026

Product Citations:
1. Lalevée, N., et al. Current Biology, 16(15), 1502-1508, 2006.