High Fidelity, Ready-to-go pre-mix

Catalogue Numbers:
BIO-25027 100 Reactions
BIO-25028 500 Reactions

Features
         • The same high fidelity and high yield as ACCUZYME™ DNA Polymerase
         • Convenient pre-mixed, pre-optimized 2x solutions
         • Reduced risk of contamination
         • Dramatically decreases the time required for reaction setup
         • Reproducible results

Applications
        • Ultra-high fidelity PCR for subsequent cloning
        • Blunt-end cloning
        • High throughput combined with high fidelity
        • DHPLC compatible (detergent-free)

Description
ACCUZYME™ Mix is a convenient ready-to-go 2x reaction mix designed for high fidelity and maximum experiment reproducibility. ACCUZYME Mix contains ACCUZYME™ DNA Polymerase, MgCl2 and ultra-pure dNTPs manufactured by Bioline. The mix is optimized and ready-to-use: the user adds only water, template and primers.
ACCUZYME Mix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured, by a reduction in the number of pipetting steps that can lead to pipetting errors.

ACCUZYME Mix Protocol
Reaction Conditions (For a 50µl reaction)
ACCUZYME™ Mix                25µl
Template and Primers               as required
Water (ddH2O)                       up to 50µl

Denature: 94-97°C
Extension: 68°C Allowing 1.5-2 minutes per Kb
For optimal resolution of PCR products, we recommend the use of Tris-Acetate EDTA (TAE) buffer for gel preparation and electrophoresis.
ACCUZYME Mix is supplied with an additional 50mM MgCl2 solution for optional optimization of reaction conditions. The table below shows the volume of MgCl2 to add to achieve different final concentrations.

   Final Magnesium                    Volume of 50mM MgCl2 to add to

concentration required                   a 50µl final reaction volume

       2.0mM                                                    0
       2.5mM                                                  0.5µl
       3.0mM                                                   1µl

This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.

Product Specifications
Batch details:
Batch No: See vial

Components
ACCUZYME Mix      100 Reactions     500 Reactions
ACCUZYME Mix        2 x 1.25ml          10 x 1.25ml
50mM MgCl2 Solution    1.2ml                    1.2ml

Storage Conditions:
ACCUZYME™ Mix can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.

Shipping Conditions:
On Dry Ice or Blue Ice

Associated Products:
Product Name                               Pack Size             Cat No
ACCUZYME DNA Polymerase    250 Units           BIO-21051
HyperLadder IV                            200 Lanes          BIO-33029
Agarose Tablets                               150g                BIO-41028
SureClean Plus                               1 x 5ml              BIO-37047