of the immunization procedure.

and purification of the IgG (7S) fraction by DEAE-chromatography.

other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess

adsorbent protein or immune complexes in the antiserum.

and double radial immunodiffusion (Ouchterlony), using various antiserum

concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin

line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine

serum and the purified albumin.

homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity

is also dependent on the concentrations of the reactants and the sensitivity of the assay

arrangement. This antiserum fraction has been tested for cross-reactivity by double radial

immunodiffusion against several species sera with the following results:

cat +

chicken -

dog +

duck -

goat ++

guinea pig ±

hamster +

horse +

human +

monkey +

mouse ±

pigeon -

rabbit -

rat ±

sheep ++

swine +

IgG protein concentration 10 mg/ml. No foreign proteins added.

appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates

with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial

carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as

catching antibody and detection reagent; in Western blotting.

Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in

ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:10,000.

storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove

insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an

aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working

dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing

should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the

same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will

not affect the performance of the product.

reconstituted at or below -20°C

reconstituted at +4°C

at least 10 years

3-5 years

7 days

only.