Immunogen: Recombinant human NKp46 protein

Isotype: Mouse IgG1 heavy chain and ? light chain

Clone: Anti-NKp46, clone n1D9, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NKp46.

Description: A natural cytotoxicity receptor (NCR), NKp46, is a glycoprotein that has two extracellular Ig-like domains followed by a ~40 residue stalk resion, a type I transmembrane domain, and a short cytoplasmic tail. NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines.

Concentration : 1mg/ml

Form: Liquid. Supplied in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

Preparation and Storage: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4? for up to one month, but store at -20? for long term storage. Avoid repeated freezing and thawing cycles.

Usage: Applications include flow cytometry, ELISA and western blot analysis. The antibody has been tested by immunofluorescent staining with flow cytometric analysis and by Western blot analysis to assure specificity and reactivity. Since application vary, however, each investigation should titrate the reagent to obtain optimal results.
* Note: this product is routinely tested on peripheral blood mononuclear cells(PBMCs) or natural killer(NK) cells. The histogram is an example of the flow cytometric analyses