This is an ELISA (Enzyme Linked ImmunoSorbent Assay) kit for measurement of rat TSH (thyroid-stimulating hormone) with high sensitivity using Sandwich assay principle.
Advantage
(1) Rapid assay (total reaction time: 3 hours 50min.).
(2) A small sample volume (10µl in the standard procedure).
(3) An ecologically friendly preservative is used.
(4) Every reagent is provided in liquid form; no reconstitution is necessary.
(5) Excellent precision and reproducibility.
Components
Reagents Amounts
(A) Anti-TSH-coated plate 96 wells(8x12) / 1 plate
(B) Standard rat TSH solution (360ng/ml,
NIDDK rat TSH RP-3 equivalent) 200 µl / 1 vial
(C) Dilution Buffer solution 60ml/ 1 vial
(D) Biotin-conjugated anti-TSH 50 µl / 1 vial
(E) HRP-conjugated avidin 50 µl / 1 vial
(F) Chromogenic substrate reagent (TMB) 12ml/ 1 vial
(H) Reaction Stop Solution (1M H2SO4) 12ml/ 1 vial
(I) Concentrated Washing Buffer(10x) 100ml/ 1 bottle
Assay sample
Rat or mouse serum or plasma* : 10 µl /well (in the standard procedure)
*Please, use EDTA at the final concentration of 1mg/ml for obtaining plasma, heparin is not suitable for this assay sytem.
We recommend diluting serum or plasma with assay buffer (C) an pipetting 50 µl of the diluted sample to well.
Sample can be applied at 10 ~ 50 µl/well (5~1x).
Assay range
The range of standard curve: 0.288~36ng/ml
Important notice in the treatments
1. Treatment of assay samples
(1) Use serum samples or plasma* samples obtained by indicated method.
*Please, use EDTA at the final concentration of 1 mg/ml for obtaining plasma, heparin is not suitable for this assay system.
(2) Turbid samples or those containing insoluble materials should be centrifuged before assay and remove those materials.
(3) Measure the samples as soon as possible after sampling.
(4) It would be also convenient to dilute the assay samples first in test tubes, and pipette 50 µl of the diluted sample to a well.
2. Storage of assay samples.
If assay samples have to be stored for a long period, freeze samples and store below –35°C. Avoid repeated freezing and thawing.
3. Influence of interfering substances
If presence of interfering substances is suspected, examine by a dilution test using more than 2
points.
Statements and precaution
(1) The reagents included in this assay kit should be used only for research works.
(2) The reagent solutions of the kit should be used immediately after reconstitution.
(3) The reagents were prepared to give accurate results by their combination within the kit. So, do not combine the reagents in the kit of other lot number. Even the lot number is the same, do not mix the reagents with those that have been preserved for some period.
(4) Pipetting and dilution of the reagent solutions should be made accurately because these steps influence the assay precision.
(5) Do not dry the assay plate to avoid denaturation of the coated antibody.
(6) Measurement of the reaction time should be started from the pipetting of reagent to the first well.
(7) Prepare the standard curve in each assay.
(8) Dilution of the assay sample must be carried out using the buffer solution attached to the kit.
(9) Storage condition for the kit should be strictly followed.
(10) Be careful not to allow the reagent solutions of the kit to touch the skin and mucus membranes. Especially be careful for the stopping solution because it is 1M sulfuric acid.
(11) HRP-conjugated reagent solution, chromogenic substrate solution, and reaction stopper must be avoided tfrom contacting with any metal.
(12) In treating assay samples of animal origin, be careful for possible biohazards.
(13) As the antibody-coated plate is module type of 8wells x 12 rows, each row can be separated by a cutter and used independently.
Storage condition
Store the kit at 2~8°C. Do not freeze.
Term of validity
Six months from production. Expiration date is indicated on the container.
Unit of package
96-wells/1 plate |