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NE130/Bio - 3-Phosphoglyceric phosphokinase
| Product number |
NE130/Bio |
| Product name |
3-Phosphoglyceric phosphokinase |
| Quantity |
1 ml |
| Supplier |
NORDIC
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Gentaur offers over 1700 basic immunological reagents including antisera,
antibodies, immunoconjugates, purified proteins, controls and calibrators. In over 30 years,
Gentaur has established working reference standards which are periodically reviewed in the
light of current progress.
NORDENZYME™ is the name of a group of polyclonal immunologic reagents to enzymes.
Two different types of rabbit or sheep anti-enzyme products are offered: the isolated IgG
fractions (7S) of the specific antisera and the isolated antibodies (PAb) to the enzymes.
Products against some 180 enzymes are available either as 7S or PAb or in both forms. The
7S fractions are also available in biotinylated form.
The NORDENZYME™ products have been obtained by immunization with highly purified
enzymes.
Description Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to 3-phosphoglyceric
phosphokinase from baker’s yeast
Product code NE0130/Bio
Biological origin Rabbit
Physical form Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered
saline (PBS, pH 7.2).
Preservative No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Immunogen 3-Phosphoglyceric phosphokinase isolated and purified from baker’s yeast. Freund’s complete
adjuvant is used in the first step of the immunization procedure.
Purification The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and
ion exchange chromatography.
Identity & Specificity The reagents were evaluated for potency, purity and specificity using most or all of the
following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial
immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Cross-reactivity Cross-reactivities against enzymes of other sources may occur but have not been determined.
Physicochemical
characteristics
IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.1. No
foreign proteins added.
Marker N-Hydroxysuccinimidobiotin.
Conjugation procedure A proprietary technique for the binding to biotin is used, followed by several purification steps.
After each step activity and specificity are tested in a variety of techniques. The conjugate is
lyophilised to assure stability and long shelf life.
Intended use This product is intended for use in precipitating and non-precipitating antibody-binding assays
(such as e.g., ELISA and Western blotting and immunofluorescence or histochemical
techniques).
Directions for use Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may
be between 1/1,000 and 1/30,000.
Handling The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C;
prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water,
spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or
below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down
again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS,
pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be
stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs
upon storage, this should be removed by centrifugation. It will not affect the performance of
the product.
Packing Vial with 1 ml immunoconjugate, lyophilised from phosphate buffered saline.
Storage / shelf life Lyophilised at +4°C
reconstituted at or below -20°C
reconstituted at +4°C
at least 10 years
3-5 years
7 days
Caution This product should be handled by qualified persons only and appropriate precautions should
be taken in its handling and disposal, and of all associated materials. For in vitro laboratory
research purposes only. |
| EUR | 177 | | USD | 221 | | GBP | 142 | | DKK | 1.319 | | JPY | 29.126 | | PLZ | 603 | | SEK | 1.654 | | NOK | 1.405 |
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